Animal Health Diagnostic Center

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Salmonella Rapid Surveillance for
Veterinary Facilities


Result Interpretation

  1. Screening by real-time PCR will be under “Molecular Diagnostics” in the report. Each sample result will be in one of the following formats:
    • Negative – No Salmonella Spp. DNA was detected, and no further testing of this sample is required.
    • PositiveSalmonella Spp. DNA was detected. A cycle threshold (Ct) value between 1 and 35 will be provided, which is inversely correlated with the amount of target DNA present. This sample will automatically be cultured to determine bacterial viability, and if Salmonella is isolated, it will be serogrouped and serotyped.
    • Suspect Salmonella Spp. DNA was detected at a low level, which is unlikely to correlate with the presence of viable bacteria and may not be repeatable. A cycle threshold (Ct) value between 35 and 40 will be provided, which is inversely correlated with the amount of target DNA present. This sample will automatically be cultured, and if Salmonella is isolated, it will be serogrouped and serotyped.
    • Inconclusive – The sample contained a high level of inhibitory agents that interfered with the amplification reaction and no conclusions can be made regarding the presence or absence of Salmonella DNA in this sample. Please contact the lab (salmonella@cornell.edu) to discuss re-sampling.
  2. Confirmatory culture results with serogroup and serotype identification will be provided for any positive or suspect samples. No additional charges will apply for serotyping. The serotyping will be done after referring the isolate to NVSL. Those results will be added to the report on a later date in approximately 2-4 weeks.
  3. Antibiotic sensitivity (MIC) will be provided upon request for an additional fee.