Lab Section: Serology
Description: The WNV IgM Capture ELISA for the quantitative detection of IgM antibodies to West Nile virus (WNV) in horses and dogs induced by acute viral infection. For Canine/Canidae and Equine/Equidae only. Please provide WNV vaccination history on submission form for maximum assistance with interpretation of results.
Min Sample Volume: 200 uL
Collection Container: red top blood collection tube
Coolant: Refrigerate
Test Target: Antibody
Test Days: M, Th
Test Turnaround (business days): 3-4 days
Interpretation statement for WNV IgM ELISA
Equine:
<2.5 = Negative
2.5-3.3 = Inconclusive
> 3.3 = Positive
Serum samples submitted from horses for WNV serology can be tested for IgM antibody by the quantitative IgM capture ELISA. A positive ELISA result indicates a recent infection with WNV. IgM can be detected in horses within 7-10 days following infection and persists for up to 3 months.
Samples with a result of "inconclusive" may be indicative of a rising or waning WNV IgM response. For "inconclusive" IgM results, a second sample from the horse collected 1 to 2 weeks after the initial sample will be required to determine infection status for these animals.
This test is validated for the detection of IgM antibodies against WNV in equine serum.
