Test Search for AHDC Clients Species Keyword Test Type Lab Section All Species Amphibian Avian Bovine Camelid Canine Caprine Cervidae Equine Feline Ferret Fish Mammal Other Ovine Porcine Primate Reptile All Test Types Infectious Non-Infectious All Lab Sections Anatomic Pathology Avian Diagnostics Bacteriology Brucellosis Clinical Pathology Comparative Coagulation Endocrinology Molecular Diagnostics Parasitology Quality Milk Production Services Referral Serology Toxicology Vet Support Services Virology Each accession received will be charged a $5.00 accessioning fee, in addition to the requested testing. Fees are subject to change without notice, please call the lab if you have any questions. Contract pricing available. For information please refer to the contract billing page. All test turnaround times are listed in business days and do not include Saturdays, Sundays or holidays. Lyme Disease, Equine Multiplex Assay - EQLM Sample Details: 2 mL serum (and 1 mL CSF for neurological cases) Cost/Fee: $40.00 Comment: Indicate on the accession form if and when the horse has been vaccinated and which vaccine was used. In cases for which CSF has been submitted in addition to serum, both samples will be tested and fees will accrue on each. Samples from treated horses can be paired with the previous sample if requested. The fee will also be applied to the paired sample. Please refer to the Lyme Disease Multiplex Testing for Horses or Lyme Multiplex Questions and Answers for more information. Details Reference Ranges Test Interpretations Lab Section: Serology Description: The multiplex test is based on fluorescent bead technology that allows the simultaneous measurement of antibodies to different B. burgdorferi antigens in a single sample. Min Sample Volume: 200 uL Collection Container: red top blood collection tube Coolant: Refrigerate Test Target: Antibody Test Days: M-F Test Turnaround (business days): 1-3 days No Reference Ranges The Lyme multiplex assay determines antibodies to three antigens, called 'outer surface proteins (Osp)', of B. burgdorferi which have been shown to correlate with vaccinal antibodies, or acute and chronic stages of Lyme disease. Negative: Negative values for antibodies to all three Osp antigens are predictive that the horse is not infected. If only one or two values are in the negative range see interpretation for equivocal or positive values for the corresponding Osp antigen. Equivocal: Equivocal values can indicate very early infection or can be induced by non-specific serum reactions. If there are no positive values for any of the three Osp antigens, the horse should be retested in 2-3 weeks to confirm or exclude early infection. If one or two values are in the positive range see interpretation for positive values for that corresponding Osp antigen. Positive/ OspA (>2000 - 28,000): Positive values for antibodies to OspA are typically observed in vaccinated animals. In horses, however, antibodies to OspA also seem to rise during infection. Thus, the interpretation of results on antibodies to OspA is more complex in horses. If antibodies to OspC and/or OspF are positive, along with OspA, the horse should be considered as infected with B. burgdorferi. Positive/OspC (>1000 - 10,000): Positive values for antibodies to OspC only are indicative for early infection. Antibody values to OspA can also be elevated during early infection. Positive/OspF (>1250 - 26,000): Positive values for antibodies to OspF only are predictive for chronic infection stages. Positive values for antibodies to OspC and OspF in the same sample are indicators for an infection that occurred several weeks ago and is moving towards the chronic infection stage. FOR ADDITIONAL INFORMATION: http://ahdc.vet.cornell.edu/docs/Lyme_Disease_Multiplex_Testing_for_Horses.pdf and https://ahdc.vet.cornell.edu/docs/LymeQASerology.docx This assay is validated and accredited for the detection of antibodies to three Borrelia burgdorferi antigens in equine serum. print See Supplies for diagnostic supplies and shipping materials to meet your specimen needs. See QMPS Supplies Order Form for additional Quality Milk supplies and materials. See Shipping Instructions for packaging and labeling instructions and regulations.