<i>Cryptosporidium </i>ELISA Test Information

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Parasitology


Cryptosporidium ELISA Test Information

Test Code: CRYPTO
Test Name: Cryptosporidium ELISA

Contact Name: Dr. Mani Lejeune
Contact Telephone: 607-253-3581
E-mail: ml872@cornell.edu

Test Method: Antigen capture enzyme-linked immunosorbent assay
Sample Required: 10 grams of feces
Collection container: plastic leak proof container
Transport: Ship on cold packs
Test Day: Wednesday
Lag Time: 1-8 days
Species: All species
Results Format: Positive or Negative

Interpretation

The Alexon Cryptosporidium Monoclonal Antigen Capture Enzyme-linked Immunosorbent Assay (ELISA) is a fecal procedure designed to detect a Cryptosporidium specific antigen. This procedure is done in parallel with a double centrifugation concentration flotation procedure for Cryptosporidium oocysts and any other parasites that may be present in the fecal sample.

The results of this test can be interpreted as follows:

Cryptosporidium ELISA Positive/Flotation Positive   
The animal is infected with Cryptosporidium.

Cryptosporidium ELISA Positive/ Flotation Negative
The animal may be infected with Cryptosporidium but is shedding oocysts below the limits of detection by flotation. Alternatively, the ELISA is a false positive that may be seen most frequently when the results are in the low positive range. To resolve the issue, collect a second sample for analysis.

Cryptosporidium ELISA Negative/ Flotation Positive 
The animal may be infected with Cryptosporidium but is producing antigen below the limits of detection by ELISA. Alternatively, the ELISA is a false negative. To resolve the issue, collect a second sample for analysis.

Cryptosporidium ELISA Negative/ Flotation Negative 
The animal is not infected with Cryptosporidium.